Isoflurane-induced depolarization of neural mitochondria increases with age

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Abstract

Background and objectives

The mitochondrial membrane potential (ΔΨm) drives the three fundamental functions of mitochondria, namely adenosine triphosphate (ATP) generation, Ca2+ uptake/storage, and generation/detoxification of ROS. Isoflurane depolarizes neural mitochondria. The sensitivity for general anesthetics increases with age, but the mechanism for this age-related sensitivity is still unknown. We compared the effect of isoflurane on [Ca2+]i and ΔΨm in isolated pre-synaptic terminals (synaptosomes) from neonatal, adolescent, and adult rats and the influence of interventions in the respiratory chain was assessed.

Methods

Synaptosomes were loaded with the fluorescent probes fura-2 ([Ca2+]i) and JC-1 (ΔΨm) and exposed to isoflurane 1 and 2 minimum alveolar concentration (MAC). The effect on the electron transport chain was investigated by blocking complexes I and V.

Results

In neonatal rats isoflurane had no significant effect on ΔΨm. In adolescent and adult synaptosomes, however, isoflurane 1 and 2 MAC decreased ΔΨm. Isoflurane 2 MAC increased [Ca2+]i in neonatal and adolescent rats, but not in adult synaptosomes. In Ca2+-depleted medium, isoflurane still decreased ΔΨm, while [Ca2+]i remained unaltered. By blocking complex V of the respiratory chain, the isoflurane-induced mitochondrial depolarization was enhanced in all age groups. Blocking complex I depolarized the mitochondria to the same extent as isoflurane 2 MAC, but without any additive effect.

Conclusions

The depolarizing effect of isoflurane on neural mitochondria is more pronounced in the adolescent and adult than in neonatal synaptosomes. The increased mitochondrial sensitivity with age seems to be related to the reversed function of the ATP synthase of the electron transport chain.

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