Antioxidants, or anti-oxidant agents, have attracted a great deal of attention in recent years because of their roles in prevention of chronic diseases and utilization as preservatives in food and cosmetics. In this study, we clearly demonstrated that zebrafish recombinant phosvitin (rPv) is an antioxidant agent capable of inhibiting the oxidation of the linoleic acid, and scavenging the 2,2-diphenyl-1-picrylhydrazyl radical. We also showed that zebrafish rPv is a cellular antioxidant capable of protecting radical-mediated oxidation of cellular biomolecules. Importantly, zebrafish rPv is non-cytotoxic to murine macrophage RAW264.7 cells. It is the first report that showed the antioxidant activities of Pv in fishes, suggesting that zebrafish Pv can be an important antioxidant, which can be used as preservatives in food and cosmetics and even as supplementary mediator in different diseased states.