The Introduction of the 9α-Hydroxy Group into Androst-4-en-3,17-dione Using a New Actinobacterium Strain

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Abstract

A 9α-hydrolase activity of a new actinobacterium strain identified as Rhodococcus erythropolis based on the analysis of a 16S rRNA gene sequence (1417 nucleotides) towards androst-4-en-3,17-dione (AD) was studied. In the presence of glucose in the medium, this strain completely transformed AD (4–20 g/l) into 9α-hydroxy-AD over 20–48 h. This culture was able to grow and perform AD 9α-hydroxylation at a concentration of dimethyl formamide up to 9%. Crystalline 9α-hydroxy-AD was isolated with a yield of over 90%.

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