The aim of the present study was to examine the influence of age and gender on the pro/antioxidant balance in young healthy subject aged 20–40 years. The individuals were divided into women: aged 20–25 years (group A), aged 32–39 years (group B), and men aged 20–25 years (group C). In blood, the metals, markers of oxidative stress and antioxidants were estimated. The Cu level and Cu/Zn ratio in group B was higher than in group A. There were no differences in metals concentrations between groups A and C. The similar levels of malondialdehyde concentrations and gamma-glutamyl transpeptidase activities were observed in all groups. The decrease in glutathione reduced and glutathione peroxidase was in group B than in group A. The higher glutathione reduced, lower glutathione oxidized concentrations and glutathionedependent enzymes (glutathione reductase and glutathione peroxidase) were observed in group C when compared to group A. The superoxide dismutase and catalase activities were higher in groups B and C than in group A.
The age and gender have an influence on the pro/antioxidant status in healthy people aged 20–40 years. The main antioxidants responsible for the removal of free radicals in men aged 20–25 years and women aged 32–39 years are superoxide dismutase and catalase, while the glutathione peroxidase activity declines. The detoxification being mediated through glutathione is weakened in women aged 32–39 years.