Effects of Single-Nucleotide Polymorphisms in the TLR7 and TLR9 Genes of Asthmatic Children

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Abstract

Abstract.

Background. To reveal the status of TLR7 and TLR9 SNPs among children and identify correlations between TLR7/TLR9 and asthma susceptibility and phenotypes. Method. Genomes were isolated from the blood samples of 100 asthmatic and 104 healthy children. To determine the status of TLR7 (rsl79009 and rs5935436) and TLR9 (rsl87084 and rs5743836) SNPs, DNA was amplified by PCR and subjected to Sanger sequencing. T and C polymorphisms at nucleotide position -1486 (TLR9 rsl87084) was discovered in both groups. Results. The frequencies of TT, TC, and CC genotypes at position -1486 were significantly different when the research group was compared to the control group. There were also statistically significant differences in the genotypes at position -1486 based on age and gender following stratification analysis (p<0.05). The rsl79009 (T→C) polymorphism of TLR7 was observed in both groups. There was a statistically significant difference in the genotype frequencies at locus rsl79009 between the two groups (p<0.05). In addition, there were statistically significant differences in the genotype frequencies at locus rsl79009 based on age and gender following stratification analysis (p<0.05). Conclusion. Significant correlations between TLR9/TLR7 SNPs and pediatric asthma were identified. The genotypes at TLR7 rsl79009 and TLR9 rsl87084 were significantly different between the two groups.

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