The aim of this study is to evaluate long-term safety and efficacy of in-vivo transcleral Fine Needle Aspiration Biopsy (FNAB) for cytogenetic testing of posterior uveal melanoma (UM).Methods:
One hundred thirty-nine consecutive patients affected by UM underwent in-vivo 25-G transcleral FNAB just before brachitherapy. Sampled material underwent both FISH (chromosome 3 and 6) and MLPA analysis using standard procedures. Follow-up was longer than 36 months.Results:
The use of 25-G transcleral FNAB appears a long-term safe and effective procedure for in-vivo cytogenetic testing of posterior uveal melanoma. Combined analysis of both arms of uveal melanoma bifurcated pathway (-3 and +6p) increase predictive value of FISH technique. MLPA allows obtaining more information than standard FISH in UM prognostication (partial chromosomes deletion).Conclusions:
The biological and prognostic value of partial chromosome 3 deletion, as well as others subtle chromosomes alterations or complex MLPA results, remains unclear.