The superfamily TRP (Transient Receptor Potential Channels), including subfamily TRPV (1-6) channels, play an important role in regulation of eye homeostasis and development of ophthalmic pathology (Zhang F. et.all, 2007). The purpose of the paper is to study the possibility of suppression of TRPV1 channels activity that can promote braking of pathological processes in the cornea and the retina.Methods
In experiment the action of laminaria dry extract is investigated on the stable line of CHO cells, expressing the receptor of the rat TRPV1 (rTRPV1). This line was received with use of the T-REx System (Invitrogene). kDNA coding rTRPV1, was cloned in an induced expressed vector of pcDNA4/TO. The expression of TRPV1 was induced by addition of tetracycline on cellular environment (1mg/ml) in 24 hours prior to testing. The selected monoclones with the greatest level of an expression of TRPV1 were used. The fluorescent analysis was carried out with the automatic system of dispensing of NOVOstar liquids (BMG LABTECH). Change of cellular fluorescence (λex=485 nm, λem=520 nm) before and after stimulation of cells agonist TRPV1 - capsaicin (400 nM) in the presence of various concentrations of laminariadry extract was studied. Measurements were performed at pH7.4 and 37 °C.Results
In experiment it was established that an application of the laminaria in concentration of 4-5 mg/ml on cells with an expressed receptor of TRPV1 considerable falling (30-50%) Ca2+ response of cells at a single activation of cells with the capsaicin.Conclusion
Thus, the extract of laminaria shows a considerable inhibiting activity in relation to TRPV1 to channels. It may be one of the ways in the treatment of ophthalmic pathology.