Effect of chromogranin A-derived vasostatin-1 on laser-induced choroidal neovascularization in the mouse

    loading  Checking for direct PDF access through Ovid

Abstract

Purpose:

To verify the effect of vasostatin-1 (VS-1), an anti-angiogenic fragment of chromogranin A, in the prevention of choroidal neovascularization (CNV) in an established mouse model of laser-induced ocular neovascularization.

Methods:

Bruch's membrane, the innermost layer of the choroid, was broken by laser photocoagulation in C57/Bl6 mice, to induce CNV. Mice were then treated daily for 14 days by intraperitoneal injection of VS-1 or vehicle (6 mice/group). CNV and vascular leakage were measured at three time-points (day 0, 7 and 14)in vivoby spectral domain optical coherence tomography (OCT) and fluorescein angiography (FA).Ex vivoanalysis of CNV was also performed at day 14 by confocal microscopy analysis of dextran-perfused choroidal flat-mounts.

Results:

In vivoanalyses showed that VS-1 significantly reduced CNV at day 14 (p = 0.03) and vascular leakage at day 7 (p = 0.01) and 14 (p = 0.04).Ex vivoconfocal microscopy analysis of CNV performed on dextran-perfused choroidal flat-mounts at day 14 confirmed the protective activity of VS-1 (p = 0.01). A significant correlation between the results ofin vivoandex vivoanalyses of CNV was also observed (p = 0.001,R2 = 0.81).

Conclusion:

The results indicate that VS-1 can prevent CNV and vascular leakage in a mouse model of ocular neovascularization, suggesting that this polypeptide might have therapeutic activity in human ocular diseases that are complicated by neovascularization or excessive vascular permeability.

Related Topics

    loading  Loading Related Articles