Chitosan Scaffolds with Unidirectional Microtubular Pores for Large Skeletal Myotube Generation

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3D, uniaxially aligned, microtubular, porous chitosan scaffolds

prepared via directional freeze-drying support the formation of large anisotropic myotubes. Myotubes of ˜550 μm long and ˜50 μm diameter are grown in the scaffolds after two weeks of culture. Porous chitosan scaffolds of varying pore architectures and mechanical properties can be readily produced using this fabrication process for engineering various anisotropic tissues.

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