Endotoxin, Interleukin-1beta, Interleukin-6, or Tumor Necrosis Factor-Alpha Do Not Acutely Stimulate Isolated Murine Myometrial Contractile Activity

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Abstract

OBJECTIVES

Endotoxin, interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha have been implicated in the pathogenesis of preterm labor, but their acute effect on myometrial contractile activity is unknown. The objective of this study was to determine their effect on isolated pregnant murine myometrial contractile activity.

STUDY DESIGN

Isometric contractions were measured in myometrium isolated from pregnancy day 18 Swiss-Webster mice. Frequency, duration, amplitude, and integrated area were compared before and after the addition of endotoxin (103 and 104 ng/ml) (n = 6), interleukin-1beta (10 and 10 ng/ml) (n = 6), interleukin-6 (1 and 10 ng/ml) (n = 6), and tumor necrosis factor-alpha (1 and 10 ng/ml) (n = 6). Results were analyzed with the Wilcoxon rank-sum test.

RESULTS

The addition of endotoxin, interleukin-1beta, interleukin-6, or tumor necrosis factor-alpha did not result in a change in the contractile activity of isolated pregnant murine myometrium compared with control.

CONCLUSION

Endotoxin, interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha do not acutely increase isolated murine myometrial contractile activity. (AM J OBSTET GYNECOL 1993;169:1424-7.)

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