The main purpose of this study was the translation and analysis of psychometric properties of the Attribution Questionnaire for use in Spanish-speaking populations (AQ-27-E) and to test the dangerousness and responsibility models of mental illness stigma in a Spanish sample. The sample consisted of 439 adults from the general population of the community of Madrid (Spain). The questionnaire was translated using the translation/back-translation method. Cronbach’s alpha was used to carry out the reliability analysis, and structural equations were used to test the dangerousness and the responsibility models of stigma. Internal reliability of AQ-27-E was .855, which can be interpreted as good. However, some factors (Pity, Responsibility, and Coercion) showed limited internal consistency. Results suggest that the AQ-27-E is a standardized instrument with acceptable psychometric properties comparable to previous versions, which can be used to assess stigma in Spanish-speaking populations.