Clinical Utility of Heteroduplex Analysis of TCR Gamma Gene Rearrangements in the Diagnosis of T-Cell Lymphoproliferative Disorders

    loading  Checking for direct PDF access through Ovid


We conducted this study to assess the clinical utility of heteroduplex analysis of polymerase chain reaction (PCR) products for Tγ gene rearrangements in the diagnosis of T-cell lymphoproliferative disorders (LPDs) using a single set of consensus primers. This method was used to study 103 cases using fresh or frozen tissue. These included 62 cases of T-cell LPDs, 25 cases of B-cell LPDs, 3 cases of anaplastic large cell lymphomas of undefined lineage, and 13 cases of reactive disorders. The latter group served as the negative control group. For all cases of T-cell LPDs, the results of Tβ Southern analysis (SA) and heteroduplex PCR analysis were compared. We also studied 10 cases of postthymic T-cell lymphoma using paraffin-embedded tissue. Heteroduplex Tγ PCR was positive in 51 (89%) of 57 cases that were rearranged by Tβ SA. Eight of the 10 cases of T-cell lymphoma analyzed using paraffin-embedded tissue were positive. There were no false-positive results. These results demonstrate that this method is an effective screening test in the clinical laboratory. A positive result obviates the need to perform SA. Preliminary results suggest this method is useful using paraffinembedded tissue.

    loading  Loading Related Articles