Analysis of Vegetable Oils by High-Performance Liquid Chromatography

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A reversed-phase high-performance liquid chromatographic procedure is proposed to monitor the fatty-acid and triglyceride composition of sunflower-seed oil. The procedure uses a column packed with Diasfer-110-C18 (6 μm), a 2: 8 mixture of acetonitrile and acetone as an eluent, and refractometric detection. Analyses that use simple normalization and normalization with correction factors that take into account the difference between calculated refraction coefficients of different triglycerides are compared. It is shown that the proposed procedure can be used for the quantitative determination of total triglycerides in various vegetable oils as well.

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