Increased miR-381a-3p Contributes to Osteoarthritis by Targeting IkBα


    loading  Checking for direct PDF access through Ovid

Abstract

Abstract.Osteoarthritis (OA) affects over 100 million individuals around the world. In this study, we mainly explore the functional role of miR-381a-3p in the regulation of OA. The expression of miR-381a-3p was studied in human articular cartilage and synovium of OA. Furthermore, the level of miR-381a-3p was analyzed in the OA rat model. MiR-381a-3p was overexpressed or inhibited with specific miR mimics or inhibitors. RT-PCR was applied to determine the levels of inflammatory factors such as TNFα, COX-2, iNOS, IL-6, and IL8. Bioinformatic predictions were applied to determine the possible target genes. Dual luciferase reporter assay was used to explore whether IκBα was the target gene of miR-381a-3p. The level of miR-381a-3p was significantly increased in the human articular cartilage and synovium of OA compared with normal control. Meanwhile, miR-381a-3p was enhanced in the OA rat model. Overexpression of miR-381a-3p significantly enhanced the levels of TNFα, COX-2, iNOS, IL-6 and IL8. IκBα was predicted as the target gene of miR-381a-3p. Dual luciferase reporter assay showed that miR-381a-3p could significantly decrease the luciferase activity of pmirGL- IκBα-3'UTR. In summary, enhanced miR-381a-3p expression contributed to the injury of OA mainly by inhibiting the expression of IκBα.

    loading  Loading Related Articles