High prevalence of CTX-M β-lactamase-producing Enterobacteriaceae in stool specimens obtained from healthy individuals in Thailand


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Abstract

ObjectivesTo determine the prevalence of CTX-M β-lactamase-producing Enterobacteriaceae in stool specimens obtained from healthy individuals in a rural area of Thailand.MethodsBacteria in stool specimens were screened for extended-spectrum β-lactamase (ESBL) production on McConkey agar with cefotaxime and confirmed by the double-disc synergy test. Genetic detection and genotyping of CTX-M-type ESBL was performed by PCR with bacterial DNA extracted from isolates.ResultsA markedly high number (82 of 141, 58.2%) of the specimens showed the presence of CTX-M β-lactamase-producing Enterobacteriaceae, as confirmed by both phenotypic and genetic examinations. The majority of the CTX-M β-lactamase-producing bacteria were Escherichia coli (85.1%).ConclusionsThe study revealed the wide dissemination of CTX-M β-lactamase-producing Enterobacteriaceae in the healthy population.

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