Quantification ofUreaplasma urealyticumDNA in the amniotic fluid from patients in PTL and pPROM and its relation to inflammatory cytokine levels

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Abstract

Objective

To study the effect of the amniotic fluid quantity of

Ureaplasma urealyticum

DNA on inflammatory response levels in women with preterm labor (PTL) and preterm prelabor rupture of membranes (pPROM).

Design

A prospective multi-center follow up study.

Setting

Sahlgrenska University Hospital, Göteborg, Sweden and Turku University Hospital, Turku, Finland.

Sample

Eleven U. urealyticum positive samples obtained after transabdominal amniocenteses in 197 women presenting with PTL and pPROM.

Methods

The U. urealyticum positive samples were analyzed with real-time polymerase chain reaction, using the Lightcycler instrument with primers specific for U. urealyticum 16 S rDNA. The amniotic fluid samples were analyzed for tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β and IL-10 with enzyme-linked immunosorbent assays.

Main outcome measures

Correlation between U. urealyticum DNA concentrations in the amniotic fluid and inflammatory cytokine levels.

Results

The concentrations of U. urealyticum DNA varied between 0.024 and 934 μg/mL. A significant correlation between U. urealyticum DNA and TNF-α level was observed. No correlation with the other cytokines was found. Women with PTLhad higher levels of U. urealyticum DNA and a different cytokine pattern than women with pPROM.

Conclusions

U. urealyticum in the amniotic fluid induces an inflammatory reaction in a dose dependent manner and the quantity of U. urealyticum DNA is well correlated with the level of the inflammatory cytokine TNF-α.

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