The purpose of the present study was to assess the temporal variation of the facial electroneurography (ENoG) parameters recorded from the nasolabial crease and to compare it to the temporal oscillations of oral and facial cutaneous temperatures. Twelve healthy individuals were examined at 4-hour intervals for 2 consecutive days. Twenty-four-hour rhythms of oral and facial cutaneous temperatures were found in all subjects (troughs on average at 4:20 and 4:00 am, respectively). Significant 24-hour oscillations of ENoG amplitude were found in all subjects, which were positively correlated to the oscillations of oral and facial cutaneous temperatures (trough on average at 2:40 am). The compound action potential (CAP) latency fluctuated during the day and was negatively correlated with local temperatures. However, these fluctuations and correlations did not reach statistical significance. We also found a significant reduction of ENoG amplitude in 5 patients with artificial cooling of the face near the facial nerve. Also, there was a small increase in CAP latency with facial cooling. Interpreting ENoG recordings in accordance with oral temperature and recording time of day reduce the intertest variation.