Identification of waterborne bacteria by the analysis of 16S–23S rRNA intergenic spacer region

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Abstract

Aim

In this study, we evaluated, the use of universal primers, specific for the 16S-23S rRNA intergenic region, to detect and identify nine species that are of high interest for the microbiological control of water.

Methods and Results

The analysis of the fragments was carried out using a High Resolution acrylamide/bisacrylamide gels in a fluorescent automated DNA sequencer. The results showed specific profiles for each of the nine species but this technique failed to detect simultaneously micro-organisms in samples containing a mixed population.

Conclusion

Nevertheless, the electrophoretic profiles obtained provided a very useful tool for the rapid and specific identification of water isolates.

Significance and impact of the study

A possible new methodology for a rapid identification of pathogens in water.

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