Formation of Extracellular Enzyme Systems during the Growth of Geotrichum candidum 3C on Cell Walls Isolated from Cereal Grain Coats


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Abstract

The activities of extracellular systems of hemicellulases, pectinases, and cellulases was studied during a 72-h cultivation of Geotrichum candidum 3C. The culture was grown on a medium containing 3% cell walls isolated from wheat grain coats, which served as the sole carbon source. Enzymes catalyzing the degradation of pectin substances (beet pectin, α-L-arabinan, and 1,4-β-D-galactan), as well as β-D-galactosidase and α-L-arabinofuranosidase involved in their hydrolysis, were formed first (4 h after the beginning of cultivation). Enzymes hydrolyzing 4-O-methyl-α-D-glucurono-β-D-xylan and sodium carboxymethyl xylan were also found in the culture liquid after 4 h of fungal growth. The contents of pectin-degrading and xylanolytic enzymes reached their maximum levels after 52–56 and 72 h of growth, respectively. Cellulolytic enzymes were detected after 8–28 h of cultivation. Enzymes degrading α-D-galacto-β-D-mannan were found 24 h after the beginning of growth; their content was maximum after 72 h of cultivation.

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