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The effects of ambiol, a new growth regulator, on the formation of leaves and roots in parent and defensin-gene-transformed regenerants of potato Solanum tuberosum L. (cultivar Desire) were studied. Various concentrations of ambiol induced differences in morphogenetic parameters between parent and transgenic plants. In some cases, ambiol caused the formation of shoots without leaves or with rudimentary leaves. The data suggest that features of root and leaf formation in parent and transgenic regenerants induced by ambiol are due to changes in hormone balance in transgenic plants caused by expression of the defensin gene and the effect of ambiol on the plant hormonal balance.