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Growth and lipogenesis of the fungus Mucor lusitanicus 306D, producing γ-linoleic acid, was studied under various conditions of nitrogen and carbon nutrition. Media containing food-industry wastes, such as maize extract, molasses, and protein hydrolysate, were used. The content of γ-linoleic acid was higher when carbohydrates (glucose and molasses) were used as carbon sources and urea was used as a nitrogen source. At a high glucose concentration (100 g/l), fed batch cultivation ensured high contents of γ-linoleic acid in lipids (1 g/l). After extraction of lipids, the fungus biomass was 42% protein, containing all essential amino acids. A defatted biomass was shown to be effectively assimilated by minks.