Isolation and Purification of Bovine Testicular Hyaluronidase

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We developed a one-step method for the chromatographic purification of hyaluronidase extract on Sepharose Blue, which produced the purified enzyme with a high yield (95%). Purification of hyaluronidase allowed us to obtain biological preparations standardized in protein composition and enzyme activity, which are useful for basic and applied studies.

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