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The purpose of this article was to assess validity of the regional Disabilities of Arm, Shoulder, and Hand (QuickDASH) and Short-Form 12 (SF-12) for surveillance purpose. We compared the predictive, discriminate, and concurrent validity of the QuickDASH and SF-12 among 231 workers with specific clinical diagnoses of neck or upper extremity musculoskeletal disorders (UEMSDs) and 175 workers with symptoms only. Compared to those with symptoms only, the odds of being any neck or UEMSD case were 1.45 (95% confidence interval [95% CI]: 1.24–1.70) and 0.66 (95% CI: 0.48–0.91) with every 10-point increase in QuickDASH disability and physical component scale (PCS-12) scores, respectively. The clinical cases had significantly higher QuickDASH disability (23.0 vs. 14.3, p < 0.0001) and lower PCS-12 scores (44.8 vs. 47.3, p = 0.0133) than those with symptom only. The QuickDASH disability scores were moderately correlated with the PCS-12 scores (rho = −0.40) among the clinical cases. Either QuickDASH or PCS-12 can be used as a simple surveillance tool in an active working population.