Lung Mechanics and Histology During Sevoflurane Anesthesia in a Model of Chronic Allergic Asthma

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Abstract

BACKGROUND:

There are no studies examining the effects of sevoflurane on a chronically inflamed and remodeled airway, such as that found in asthma. In the present study, we sought to define the respiratory effects of sevoflurane in a model of chronic allergic asthma. For this purpose, pulmonary mechanics were studied and lung morphometry analyzed to determine whether the physiological modifications reflected underlying morphological changes.

METHODS:

Thirty-six BALB/c mice (20–25 g) were randomly divided into four groups. In OVA groups, mice were sensitized with ovalbumin and exposed to repeated ovalbumin challenges. In SAL groups, mice received saline using the same protocol. Twenty-four hours after the last challenge, the animals were anesthetized with pentobarbital sodium (PENTO, 20 mg/kg i.p.) or sevoflurane (SEVO, 1 MAC). Lung static elastance (Est), resistive (ΔP1) and viscoelastic/inhomogeneous (ΔP2) pressure decreases were analyzed by an end-inflation occlusion method. Lungs were fixed and stained for histological analysis.

RESULTS:

Animals in the OVASEVO group showed lower ΔP1 (38%), ΔP2 (24%), and Est (22%) than animals in the OVAPENTO group. Histology demonstrated greater airway dilation (16%) and a lower degree of alveolar collapse (25%) in the OVASEVO compared with OVAPENTO group. ΔP1 was lower (35%) and airway diameters larger (12%) in the SALSEVO compared with SALPENTO group.

CONCLUSION:

Sevoflurane anesthesia acted both at airway level and lung periphery reducing (ΔP1 and ΔP2 pressures, and Est in chronic allergic asthma.

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