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Bioactive glasses (BG) is one of the well-known materials that used as dental and bone implants, for this reason it is always interesting for researchers has been to increase BG efficiency in the bone tissue engineering. The aim of this study was to evaluate the osteoinductivity of BG different composition nanoparticles with SiO2–CaO–P2O5. The 45S, 58S, and 63S compositions were prepared via the sol–gel technique. Characterization techniques such as x-ray diffraction, field emission scanning electron microscopy (FE-SEM), and laser Doppler electrophoresis (LDE) were used. The osteoinductive capacity of prepared nanoparticles was investigated using unrestricted somatic stem cells (USSC). The particle size of the samples with an amorphous structure mainly ranged less than 40 nm. The zeta potential was negative for all compositions in distilled water at pH 7.4. Bioactive glass nanoparticles were shown to support proliferation of USSC, as shown by microculture tetrazolium (MTT) assay. During osteogenic differentiation, significantly highest values of alkaline phosphatase (ALP) activity and biomineralization were observed on 45S BG. Subsequently, these markers were measured in higher amounts in USSC on 58S and 63S BG compared with tissue culture polystyrene. The nanometric particle size, osteoinductivity, and negative zeta potential make this material a possible candidate for bone tissue engineering applications.