Aortic Smooth Muscle Cells in a Three-Dimensional Collagen Lattice Culture: Evidence for Posttranslational Regulation of Collagen Synthesis

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Abstract

Aortic smooth muscle cells were cultivated as monolayers on plastic or within collagen lattices with lowand high-serum supplementation, and the expression of mRNAs specific for pro alpha 1 (I) and pro alpha 1 (HI) collagen were studied by slot blot hybridization. The steady-state levels of pro alpha 1 (I) and pro alpha 1 (HI) collagen mRNA of cells within collagen lattices were found to be higher than those grown on plastic, although the production of collagen was lower. The degradation of pro alpha 1 (I) and pro alpha 1 (HI) collagen mRNAs as revealed in the presence of actinomycin D was not affected by culturing the cells within a collagen lattice. In vitro translation assays of mRNAs of monolayerand lattice-cultured cells showed no differences in translatability. These data suggest the involvement of posttranslational control of collagen production in collagen lattice-cultured smooth muscle cells.

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