Disposable pipette extraction for the simultaneous determination of biperiden and three antipsychotic drugs in human urine by GC–nitrogen phosphorus detection

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Abstract

Background:

Disposable pipette extraction with reversed-phase sorbent is proposed for the fast and simple GC determination of the antipsychotic drugs chlorpromazine, olanzapine, clozapine and biperiden – an anticholinergic drug – in human urine. The method was validated and successfully applied to postmortem urine samples. The analytical run was 11 min and lidocaine was used as the internal standard.

Results:

The developed method showed good linearity, over the range of 0.34 to 5 ng/µl, for all compounds. The within-day and between-day precision and accuracy assays revealed values ≤15%, while the recoveries ranged from 85 to 120%. LOD and LOQ ranged from 0.28 to 0.42 ng/µl and from 0.85 to 3.58 ng/µl, respectively.

Conclusion:

The developed method is a user-friendly technique, which is simple and fast.

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