Expansion of a (GA) Dinucleotide at a Microsatellite Locus Associated with Domestication in Rice


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Abstract

Microsatellites undergo rapid changes over short evolutionary time periods which can be phylogenetically informative in related species. Here we show the repeat unit expansion of a (GA)n-type microsatellite in the process of cultivation of rice from its wild ancestors. We amplified a microsatellite locus harboring (GA)n repeats from several wild and cultivated rices. Sequencing revealed an increase in repeat number from 14 in distantly related wild rice species to 24 in the widely grown present-day indica rice cultivars.

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