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Transformation of 2,4-dinitrotoluene (2,4-DNT) by Alcaligenes JS867 under varying degrees of oxygen limitation was examined. Complete 2,4-DNT removal was observed under oxygen excess with near stoichiometric release (83%) of nitrite. Average kinetic parameters were estimated based on a dual-Monod biokinetic model with 2,4-DNT and O2 as growth limiting substrates. The negative impact of nitrite accumulation on the reaction rate was adequately described by inclusion of a noncompetitive inhibition term for NO2−. Under aerobic conditions, μmax, KsDNT, and KiNO were 0.058(0.004) hr−1, 3.3(±1.3) mg 2,4-DNT/L, and 1.2(±pm0.2) hr−1, respectively. At increasing oxygen limitation, rates of 2,4-DNT disappearance and nitrite production decreased and incomplete removal of 2,4-DNT commenced. JS867 was able to use NO2−as a terminal electron acceptor when grown on glucose or succinate under anaerobic conditions. However, during growth on 2,4-DNT and under O2-limited conditions, JS867 did not use released nitrite as electron acceptor. The nearly constant molar ratios of DNT removed over NO2−released under various degrees of oxygen limitation suggested that oxygenolytic denitration pathways continued. No evidence of nitroreduction was obtained under the examined oligotrophic conditions. JS867 displayed a high affinity for oxygen consumption with KSO2 value of 0.285(±0.198) mg O2/L. Our results indicate that under oligotrophic conditions with 2,4-DNT as dominant carbon source, oxygen availability and nitrite accumulation may limit 2,4-DNT biomineralization, but the accumulation of reduced 2,4-DNT transformation products will be small.