Measurement of genome-wide RNA synthesis and decay rates with Dynamic Transcriptome Analysis (DTA)

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Abstract

Summary: Standard transcriptomics measures total cellular RNA levels. Our understanding of gene regulation would be greatly improved if we could measure RNA synthesis and decay rates on a genome-wide level. To that end, the Dynamic Transcriptome Analysis (DTA) method has been developed. DTA combines metabolic RNA labeling with standard transcriptomics to measure RNA synthesis and decay rates in a precise and non-perturbing manner. Here, we present the open source R/Bioconductor software package DTA. It implements all required bioinformatics steps that allow the accurate absolute quantification and comparison of RNA turnover.

Availability:DTA is part of R/Bioconductor. To download and install DTA refer to http://bioconductor.org/packages/2.10/bioc/html/DTA.html

Contact:schwalb@lmb.uni-muenchen.de

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