Chainy: an universal tool for standardized relative quantification in real-time PCR

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Chainy is a cross-platform web tool providing systematic pipelines and steady criteria to process real-time PCR data, including the calculation of efficiencies from raw data by kinetic methods, evaluation of the suitability of multiple references, standardized normalization using one or more references, and group-wise relative quantification statistical testing. We illustrate the utility of Chainy for differential expression and chromatin immunoprecipitation enrichment (ChIP-QPCR) analysis.

Availability and Implementation:

Chainy is open source and freely available at


Supplementary information:

Supplementary data are available at Bioinformatics online.

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