Threshold-seq: a tool for determining the threshold in short RNA-seq datasets

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We present ‘Threshold-seq,’ a new approach for determining thresholds in deep-sequencing datasets of short RNA transcripts. Threshold-seq addresses the critical question of how many reads need to support a short RNA molecule in a given dataset before it can be considered different from ‘background.’ The proposed scheme is easy to implement and incorporate into existing pipelines.

Availability and Implementation:

Source code of Threshold-seq is freely available as an R package at:


Supplementary information:

Supplementary data are available at Bioinformatics online.

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