1Department of Molecular and Human Genetics, Baylor College of Medicine, Howard Hughes Medical Institute, Houston, TX, USA and 2Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital, Houston, TX, USA and 3Department of Pediatrics and 4Baylor College of Medicine, Howard Hughes Medical Institute, Houston, TX, USA
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Summary:We present a user-friendly, cloud-based, data analysis pipeline for the deconvolution of pooled screening data. This tool, CRISPRcloud, serves a dual purpose of extracting, clustering and analyzing raw next generation sequencing files derived from pooled screening experiments while at the same time presenting them in a user-friendly way on a secure web-based platform. Moreover, CRISPRcloud serves as a useful web-based analysis pipeline for reanalysis of pooled CRISPR screening datasets. Taken together, the framework described in this study is expected to accelerate development of web-based bioinformatics tool for handling all studies which include next generation sequencing data.Availability and implementation:http://crispr.nrihub.orgContact:email@example.comSupplementary information:Supplementary data are available at Bioinformatics online.