Metabolic labelling of RNA is a well-established and powerful method to estimate RNA synthesis and decay rates. The pulseR R package simplifies the analysis of RNA-seq count data that emerge from corresponding pulse-chase experiments.Results
The pulseR package provides a flexible interface and readily accommodates numerous different experimental designs. To our knowledge, it is the first publicly available software solution that models count data with the more appropriate negative-binomial model. Moreover, pulseR handles labelled and unlabelled spike-in sets in its workflow and accounts for potential labeling biases (e.g. number of uridine residues).Availability and implementation
The pulseR package is freely available at https://github.com/dieterich-lab/pulseR under the GPLv3.0 licence.Contact
firstname.lastname@example.org or email@example.comSupplementary information
Supplementary data are available at Bioinformatics online.