Radical Scavenging Activity of Ribonuclease Inhibitor from Cow Placenta

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Cow placenta ribonuclease inhibitor (CPRI) has been purified 5062-fold by affinity chromatography, the product being homogeneous by sodium dodecyl sulfate-gel electrophoresis. The chemiluminescence technique was used to determine the radical scavenging activities of CPRI toward different reactive oxygen species (ROS) including superoxide anion (O−⋅2), hydroxyl radical (OH⋅), lipid-derived radicals (R⋅), and singlet oxygen (1O2). CPRI could effectively scavenge ⋅, OH⋅, R⋅, and 1O2 at EC50 of 0.12, 0.008, 0.009, and 0.006 mg/ml, respectively. In addition, the radical scavenging activities of CPRI were higher than those of tea polyphenols, indicating that CPRI is a powerful antioxidant.

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