Design and Construction of a Preparative-Scale Dynamic Field Gradient Focusing Apparatus

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Abstract

A linear model is used to show that dynamic field gradient focusing (DFGF) can be scaled to preparative capacity, ˜O (10 mgs). This paper explains how the preparative-scale DFGF apparatus was designed and fabricated. Scaled-down experiments and mathematical modeling guided material selection and design changes during construction to increase the probability that the prototype preparative-scale DFGF apparatus would perform as intended. The finished prototype successfully focused bovine hemoglobin from an initial concentration of 6.82 to 15 mg/mL and allowed for 86% recovery of injected protein.

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