Intestinal Permeability to Lactulose and Mannitol in Growing Rats with Iron-Deficiency Anemia

    loading  Checking for direct PDF access through Ovid



Iron deficiency can have nonhematological manifestations, some of which may affect the gastrointestinal tract. The aim of this study was to determine if iron-deficiency anemia in growing rats affected small-bowel permeability as assessed by the urinary ratio of lactulose and mannitol. Thirty-seven male Harlan Sprague–Dawley rats (21 d of age) were randomly divided into two groups and fed either an iron-deficient (n = 19) or an iron-sufficient diet (n = 18) that contained either 13.5 or 43.8 mg of iron/kg diet, respectively. Animals were evaluated between 25 and 38 d of dietary treatment. Intestinal permeability was assessed by measuring the lactulose/mannitol urinary ratio following administration of a solution that contained the two sugars. At the end of the study, the mean body weight of rats fed the low-iron diet was approx 95% that of the controls. The mean hemoglobin (g/dL) was significantly lower in the low-iron diet group (11.2 ± 1.4) than in the control group (16.9 ± 0.8) (p = 0.001). The liver iron concentration (μg/g) of the anemic group (41.4 ± 4.7) was also statistically (p = 0.001) lower than in the control group (116.6 ± 18.2). The lactulose/mannitol ratio was lower in the anemic rats (2.0 ± 0.7) than in the control group (2.6 ± 0.7) (p = 0.008), a finding that is not suggestive of intestinal mucosal atrophy, previously described in anemic children.

Related Topics

    loading  Loading Related Articles