Electrospray ionization mass spectrometry identification of fibrinogen Banks Peninsula (gamma 280 Tyr [arrow right] Cys): a new variant with defective polymerization

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Abstract

Summary

Fibrinogen Banks Peninsula was identified in the mother of a patient referred for investigation following recurrent epistaxis. Coagulation tests revealed prolonged thrombin and reptilase times and a decreased functional fibrinogen level. Thrombin-catalysed release of fibrino-peptides A and B was normal, and no abnormalities were detected by DNA sequencing of the regions encoding the thrombin cleavage sites in the A alpha and B beta genes. Reducing SDS-PAGE and reverse-phase HPLC analysis of purified fibrinogen chains were normal, as was electrospray ionization mass spectrometry (ESI-MS) analysis of isolated A alpha and B beta chains. However ESI-MS revealed a mass of 48 345 D for the isolated gamma chains, 31 D less than the measured mass of control chains (48 376 D). Since normal and abnormal gamma chains were not resolved, this implies a 60-62D mass decrease in 50% of the molecules. A 60 D decrease was confirmed when DNA sequencing indicated heterozygosity for a mutation of Tyr [arrow right] Cys at codon 280 of the gamma chain gene. Fibrin monomer polymerization revealed a delayed lag phase and reduced final turbidity and although factor XIIIa crosslinking of fibrinogen was normal, it is likely that this delay is due to impaired D:D self association. Recent crystallographic studies show residues gamma 280 and gamma 275 make contact across the D:D interface, suggesting a similar mechanism for the polymerization defects in fibrinogens Banks Peninsula and Tokyo II (gamma 275 Arg [arrow right] Cys).

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