To measure the activity of serum phosphodiesterase (PDE) in pre-eclampsia.Design
Nephrology Laboratory and Obstetrics Unit at São Lucas Hospital from Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, Brazil.Sample
Twenty-nine normal and 28 pregnant women with pre-eclampsia.Methods
Serum was collected from women with pre-eclampsia, at the time of diagnosis, and from gestation-matched controls. Circulating PDE activity was assessed by measuring consumption of the substrate thymidine 5′-monophosphate p-nitrophenyl ester and expressed as PDE units/l.Main outcome measures
Serum PDE Activity.Results
Mean substrate consumption was higher in pre-eclamptic condition (Vmax= 15.8 ± 1.4 versus 12.7 ± 0.9 U/L, P= 3.7 × 10−14).Conclusion
These data suggest that altered PDE activity may play a role in pre-eclampsia endothelial dysfunction.