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Research report-retrospective cohort.To examine the relationship between previous ankle injury history, Cumberland Ankle Instability Tool (CAIT) scores, and Y-Balance Test (YBT) scores.Ankle sprains are the most common injury that collegiate athletes suffer during their careers. Factors that may affect the likelihood of an ankle sprain include deficits in balance and/or coordination.Twenty-three (12 male, 11 female) university student-athletes from 9 high-risk sports with a CAIT score of 25 or lower and at least one prior ankle sprain participated. Single-limb reach distance was tested using the YBT in three reach directions; anterior (ANT), posteromedial (PM), and posterolateral (PL). A composite score was taken for each leg, combining the maximum reach distances of each direction divided by triple the leg length and multiplied by 100. The composite score from the uninjured ankle was subtracted from the injured ankle to determine the difference between the composite scores. A Pearson correlation coefficient was used to analyse the relationship between composite YBT and difference scores with the CAIT scores.For the injured ankle, there was a significant correlation between the YBT composite score (88.65%±8.1%) and CAIT score (22.69±2.9) (r=0.453, p=0.030), and between the difference of composite scores from uninjured to injured ankles (2.88%±2.17%) and CAIT scores (r=−0.518, p=0.011). Interestingly 21% (5/23) of the cohort experienced a subsequent ankle sprain within the six months following testing.There exists a positive relationship between CAIT and YBT scores; suggesting the clinical utility of both in helping to identify and differentiate those with and without chronic ankle instability. Uniquely interesting are the results from our difference score analysis whereby the higher the composite score difference, the lower the CAIT score.