The lower extremities have the highest injury rate in sport, with different types of the injury sustained in males and females. Injuries affect directly an athlete’s performance improvement by causing loss of practice time. Furthermore, treatment and rehabilitation of injuries are expensive. Injuries may cause morbidity and prevent return to play at the same level. Hence, functional movement screening, is an essential consideration in injury prevention. The purpose of this study was to com-pare the scores of the Landing Error Scoring System test (LESS) between male and female athletes. Thirteen healthy males and fourteen females volunteered were evaluated the LESS score. Data were recorded using two video cameras in sagittal and frontal planes. Prior to testing, subjects practised two trials and performed at least three trials of the jump-landing task. Three successful trials were analysed. The motion were captured and sedanaly using LESS score sheet. Independent t-test was used to compare the LESS scores between genders. The LESS score was lower in males than females (Males; 4.8±1.5, Females; 6.1±1.7, p=0.05). The four level of LESS score revealed no significant difference as the excellent (3.0±0.4, 3.3±0.4), the good (4.6±0.2, 4.6±0.4), the moderate (5.8±0.2, 6.0±0.0), and the poor level (7.1±0.2, 7.3±1.8), males and females respectively. The LESS scores showed that females have a greater lower extremity injury risk than males.