Synthesis of aromatic n-alkyl-glucoside esters in a coupled β-glucosidase and lipase reaction

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Regioselective synthesis of e.g. 6-O-phenylbutyryl-1-n-butyl-β-D-glucopyranose was achieved in 21 % yield using almond-β-glucosidase and Candida antarctica lipase B. The β-glucosidase reaction was performed in a biphasic (buffer/n-alcohol) system using free and Eupergit CTM-immobilized glucosidase. Immobilized enzyme allowed product formation even at a water content of 1 %. © Rapid Science Ltd. 1998

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