Extrachromosomal transmission of microinjected DNA to the progeny of silkwormBombyxmori

    loading  Checking for direct PDF access through Ovid


Vector DNA (pBmFRT) microinjected into the silkworm eggs (preblastoderm stage) persisted in different conformational forms throughout the period of embryonic development. Southern blot analysis confirmed the persistence of DNA as extrachromosomal copies. Slot blot analysis showed the inheritance of the injected DNA to the subsequent progenies; however the copy number of the injected vector declined in the progenies.

Related Topics

    loading  Loading Related Articles