Increase of catalytic activity of α-chymotrypsin in organic solvent by co-lyophilization with cyclodextrins

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Abstract

α-Chymotrypsin was lyophilized in the presence of 2,3,6-tri-O-methyl β-cyclodextrin, and it displayed activity 40 fold higher than free α-chymotrypsin for transesterification in acetonitrile. α-Chymotrypsin which was co-lyophilized with hydroxypropylated β- or γ;-cyclodextrins retained more than 98% of its initial activity after 6 h incubation in acetonitrile.

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