Improved conditions for the transformation by electroporation of the extracellular polysaccharide-producing methylotroph Methylobacillus sp.

    loading  Checking for direct PDF access through Ovid


The transformation efficiency of Methylobacillus sp. strain 12S, using electroporation, was unaffected by the growth phase of the cells but competent cells grown at 21 °C had a 1.9 × 103 times higher transformation efficiency than those grown at 30 °C. Heat shock treatment further increased the transformation efficiency up to 7 times.

Related Topics

    loading  Loading Related Articles