Immobilization of invertase on a new type of macroporous glycidyl methacrylate

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Invertase was immobilized via its carbohydrate moiety. The immobilized enzyme has a specific activity of 5500 IU g-1, with 45% activity yield on immobilization. In a packed bed reactor, 90% 2.5 M sucrose was converted at a flow rate of 4 bed volumes h-1. The obtained specific productivity at 40 °C of 3 kg l-1 h-1 is the best one so far. Long-term stability was 290 days in 2.5 M sucrose at 40 °C and at a flow rate of 3 bed volumes h-1.

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