Combining induced mutation and protoplasting for strain improvement ofAspergillus oryzaefor kojic acid production

    loading  Checking for direct PDF access through Ovid


By combining induced mutation, using NTG and UV irradiation, and protoplasting of a wild type strain of Aspergillus oryzae ATCC 22788, a hyper-producing strain was obtained that accumulated 41 g kojic acid l−1 in shake-flasks, which was 100-fold higher than that in the wild type strains. Similar production of kojic acid was obtained in 5 l stirred-tank fermentations.

Related Topics

    loading  Loading Related Articles