Low salt medium for lactate and ethanol production by recombinantEscherichia coliB

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Individual nutrient salts were experimentally varied to determine the minimum requirements for efficient l(+)-lactate production by recombinant strains of Escherichia coli B. Based on these results, AM1 medium was formulated with low levels of alkali metals (4.5 mM and total salts (4.2 g l-1). This medium was equally effective for ethanol production from xylose and lactate production from glucose with average productivities of 18–19 mmol l-1 h-1 for both (initial 48 h of fermentation).

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