A novel binary expression vector for production of human IL-10 inEscherichia coliandBifidobacterium longum

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Abstract

A novel expression vector (pLR) driven by hup promoter and Bifidobacterium β-galactosidase signal peptide was constructed. The pLR vector was used for the expression of the optimized human IL-10 synthetic gene in Escherichia coli and Bifidobacterium longum. In both microorganisms, rhIL-10 was in a soluble form in total extract cells. The recombinant hIL-10 was partially processed in E. coli, whereas in Bifidobacterium all rhIL-10 was found in the mature form.

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