Polyethylene glycol improves the purification of recombinant human tumor necrosis factor during ion exchange chromatography

    loading  Checking for direct PDF access through Ovid


Poly(ethylene glycol) (PEG) – either PEG 200, 600 or 4000 – when added to the buffer and feed solution gave nearly one-fold increase in the purification and about 100% recovery of recombinant human tumor necrosis factor-α during anion-exchange chromatography. The optimum concentration was 1% with the best result being achieved with PEG 200.

Related Topics

    loading  Loading Related Articles