Polyethylene glycol improves the purification of recombinant human tumor necrosis factor during ion exchange chromatography

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Abstract

Poly(ethylene glycol) (PEG) – either PEG 200, 600 or 4000 – when added to the buffer and feed solution gave nearly one-fold increase in the purification and about 100% recovery of recombinant human tumor necrosis factor-α during anion-exchange chromatography. The optimum concentration was 1% with the best result being achieved with PEG 200.

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